[Retracted] TRPM7 Upregulate the Activity of SMAD1 through PLC Signaling Way to Promote Osteogenesis of hBMSCs

Figure 6

The analysis of SMAD1 activity under inhibiting or activating TRPM7 and inhibiting or activating PLC (HSPG2). (a) (A) The activity of SMAD1 protein in cells induced with osteogenic medium for 0, 3, 7, and 14 days. (B) The relative activity of SMAD1 protein was quantified and plotted. (b) (A) The expression of P-SMAD1 protein in cells noninduced for 0, 3, 7, and 14 days. (B) Relative expression levels of P-SMAD1 protein were quantified and plotted. (c) (A) Western blot analysis of SMAD1 protein in cells induced with osteogenic medium or osteogenic medium plus shRNA-mediated knockdown of TRPM7 induced for 14 days. (B) The relative activity of SMAD1 protein was quantified and plotted. (d) (A) Western blot analysis of SMAD1 protein in cells induced with osteogenic medium or osteogenic medium supplemented with Naltriben or 2-APB induced for 14 days. (B) The relative activity of SMAD1 protein was quantified and plotted. (e) (A) Western blot analysis of SMAD1 protein in cells induced with osteogenic medium or osteogenic medium supplemented with M-3M3FBS or U73122 induced for 14 days. (B) The relative activity of SMAD1 protein was quantified and plotted. (f) (A) Western blot analysis of SMAD1 protein in cells induced with osteogenic medium supplemented with 20 μM Naltriben or 20 μM Naltriben plus 1 μM U73122 or 1 μM/mL U73122 induced for 14 days. (B) The relative activity of SMAD1 protein was quantified and plotted. (g) (A) Western blot analysis of SMAD1 protein in cells induced with osteogenic medium supplemented with 20 μM M-3M3FBS or 50 μM 2-APB plus 20 μM M-3M3FBS or 50 μM 2-APB induced for 14 days. (B) The relative activity of SMAD1 protein was quantified and plotted. , , and .

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