Quercetin Downregulates Cyclooxygenase-2 Expression and HIF-1α/VEGF Signaling-Related Angiogenesis in a Mouse Model of Abdominal Aortic Aneurysm

Figure 2

The effect of quercetin and celecoxib on histology and CD31⁺ cells of CaCl₂-induced AAAs. (a) Transverse sections of formalin-fixed aortic tissue were stained with Victoria blue (VB) (elastin in blue) and Masson’s trichrome (MT) stain (collagen in blue). Sections were immunostained for CD31 to examine the microvessel density. Arrows indicate positive CD31 immunostaining. Scale bars are 50 μm. Each section shown is a representative of five samples with similar results. (b) Percentage of area positive for elastic fibers. The area of elastic fibers in VB-stained sections was calculated by quantitative morphometric analysis with ImageJ. Results were expressed as a percentage of the area of the aortic media. (c) CD31⁺ microvessel contents in the mouse aortic wall ( in each group). vs. sham, ^# vs. control.

(a)

(b)

(c)