The Use of RNAi Technology to Interfere with Zfx Gene Increases the Male Rates of Red Deer (Cervus elaphus) Offspring

Table 2

Zfx RNAi oligonucleotide sequence with HpaI and XhoI enzyme sites.

Vector shRNA	Oligonucleotide sequence
pLL3.7 A	F: 5-TGTGAGGACTACCTTATGATTTCAAGAGAATCATAAGGTAGTCCTCACTTTTTTC-3
	R: 5-TCGAGAAAAAAGTGAGGACTACCTTATGATTCTCTTGAAATCATAAGGTAGTCCTCACA-3
pLL3.7 B	F: 5-TGACGTCTTAGCTTCTGATATTCAAGAGATATCAGAAGCTAAGACGTCTTTTTTC-3
	R: 5-TCGAGAAAAAAGACGTCTTAGCTTCTGATATCTCTTGAATATCAGAAGCTAAGACGTCA-3
pLL3.7 C	F: 5-TGAAGCAGATGTATCTGAAATTCAAGAGATTTCAGATACATCTGCTTCTTTTTTC-3
	R: 5-TCGAGAAAAAAGAAGCAGATGTATCTGAAATCTCTTGAATTTCAGATACATCTGCTTCA-3

Notes: using pLL3.7 as the vector construction of shRNA interference fragment.