The Use of RNAi Technology to Interfere with Zfx Gene Increases the Male Rates of Red Deer (Cervus elaphus) Offspring

Table 3

Conditions of PCR and oligonucleotide primer pairs.

Target gene	Gene bank	Sequence of primer (5→3)	Annealing temperature	Product (bp)
Zfx	KP257294.1	F: TATGGATTCACTCGTCAA	60°C	120
		R: CTCAGATGTAACAGAAGAAG
GAPDH	AY650282.1	F: AAGGCCATCACCATCTTCCA	60°C	80
		R: CCAGCATCACCCCACTTGA