Overexpression of miR-29a-3p Suppresses Proliferation, Migration, and Invasion of Vascular Smooth Muscle Cells in Atherosclerosis via Targeting TNFRSF1A

Figure 4

Overexpression of miR-29a-3p decreases proliferation of ox-LDL-induced vascular smooth muscle cells (VSMCs) by directly targeting TNFRSF1A in AS. (a–c) TNFRSF1A mRNA and protein expression was detected in ox-LDL-induced VSMCs with or without miR-29a-3p mimic injection using RT-qPCR and western blot. (d) Dual-luciferase report verified the direct interaction between miR-29a-3p and TNFRSF1A. (e) RT-qPCR was utilized to validate the transfection effect of pcDNA3.1-TNFRSF1A in ox-LDL-induced VSMCs. (f) CCK-8 assay was presented to examine the cell viability of ox-LDL-induced VSMCs injected with miR-29a-3p mimic and/or pcDNA3.1-TNFRSF1A. (g, h) Cell proliferation of ox-LDL-induced VSMCs was detected following transfection with miR-29a-3p mimic and/or pcDNA3.1-TNFRSF1A through colony formation assay. (i, j) Ki67 and PCNA expression in ox-LDL-induced VSMCs transfected with miR-29a-3p mimic and/or pcDNA3.1-TNFRSF1A. ; ; ; ns: no statistical significance.

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