miR-375 Inhibits the Proliferation and Invasion of Nasopharyngeal Carcinoma Cells by Suppressing PDK1
Expression levels of miR-375 and PDK1 in NPC tissues and cell lines were assessed. (a, b) qRT-PCR was performed to measure expression levels of miRNAs and PDK1 in 23 normal samples and in 38 NPC samples. miR-375 is downregulated in NPC tissue, compared with normal tissue, while PDK1 is upregulated in NPC tissue, compared with normal tissue (Student’s -test, ). (c, d) qRT-PCR was performed to evaluate miR-375 expression in the N69 epithelial normal nasopharynx cell line and NPC cell lines (CNE-1, CNE-2, C666-1, and HONE1). Conversely, levels of PDK1 were markedly increased in NPC cell lines, compared to NP69 (CNE-1, CNE-2, C666-1, and HONE1) (Student’s -test, ,,). Cell lines CNE-2 and CNE-1 showed the greatest difference in miR-375 expression levels when compared to NP69 cells and were used for subsequent analyses. Each assay was conducted in duplicate, three times. Values are presented ±s.e.m.
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