Effects of Mesenchymal Stem Cell Coculture on Human Lung Small Airway Epithelial Cells

Figure 2

Flow cytometry of small airway epithelial cells. Representative analyses of small airway epithelial (AE) cells investigated in flow cytometry. AE cells were either cultured alone without any addition of mesenchymal cells (MSC), which are the control cultures, left, or cocultured with MSCs derived from either adipose tissue (AD), middle, or bone marrow (BM), right. (a) Determination of cell viability using the eFluor 780 viability dye, which stains exclusively dead cell positive (yellow) compared to unstained controls (blue). (b) Detection of CD54-positive cells (blue), with isotype controls (red). (c) Detection of proliferating Ki67-positive cells (blue), with isotype controls (red).