Toxoplasma gondii Tyrosine-Rich Oocyst Wall Protein: A Closer Look through an In Silico Prism

Table 6

Human specific MHC-II epitopes predicted by IEDB server using HLA reference set with subsequent screening for antigenicity and allergenicity as well as IFN-γ and IL-4 induction.

Human MHC-II allele	HTL epitope	Method	Percentile rank	Antigenicity	Allergenicity	IFN-γ induction		IL-4 induction
						Result	Score	Result	Score
HLA-DRB101 : 01	FIKILLLLGLLAVTT	Consensus (comb.lib./smm/nn)	0.10	0.8754	No	Negative	61	Negative	-0.74
HLA-DRB115 : 01	FIKILLLLGLLAVTT	Consensus (smm/nn/sturniolo)	0.93	0.8754	No	Negative	61	Negative	-0.74
HLA-DPA101 : 03/DPB104 : 01	LVKEAFRHSLLPWFL	NetMHCIIpan	0.95	0.8487	Yes	Negative	-0.0405	Positive	0.02
HLA-DPA102 : 01/DPB105 : 01	MKGFIKILLLLGLLA	Consensus (comb.lib./smm/nn)	1.10	0.9498	No	Negative	71	Negative	-0.74
HLA-DPA101 : 03/DPB102 : 01	HSLLPWFLPGFFPRQ	Consensus (comb.lib./smm/nn)	1.40	1.2901	No	Positive	1	Negative	0.15
HLA-DPA101 : 03/DPB104 : 01	KEAFRHSLLPWFLPG	NetMHCIIpan	1.50	0.7021	Yes	Positive	0.0844	Negative	0.07
HLA-DPA103 : 01/DPB104 : 02	GFIKILLLLGLLAVT	Consensus (comb.lib./smm/nn)	1.60	0.4212	No	Negative	71	Negative	-0.74
HLA-DPA101 : 03/DPB104 : 01	HSLLPWFLPGFFPRQ	NetMHCIIpan	1.60	1.2901	No	Positive	1	Negative	0.15
HLA-DPA103 : 01/DPB104 : 02	MKGFIKILLLLGLLA	Consensus (comb.lib./smm/nn)	1.60	0.9498	No	Negative	1	Negative	-0.74
HLA-DQA101 : 01/DQB105 : 01	FRHSLLPWFLPGFFP	Consensus (comb.lib./smm/nn)	1.90	1.5483	No	Negative	-0.0952	Negative	0.14
HLA-DPA101 : 03/DPB104 : 01	EAFRHSLLPWFLPGF	NetMHCIIpan	2.00	0.9177	Yes	Negative	-0.0048	Negative	0.08
HLA-DQA101 : 01/DQB105 : 01	HSLLPWFLPGFFPRQ	Consensus (comb.lib./smm/nn)	2.00	1.2901	No	Positive	1	Negative	0.15
HLA-DPA102 : 01/DPB101 : 01	RHSLLPWFLPGFFPR	Consensus (comb.lib./smm/nn)	2.30	1.1923	No	Positive	0.0806	Negative	0.15
HLA-DRB103 : 01	KLSFIECDCRKKRVR	Consensus (smm/nn/sturniolo)	2.40	1.1250	No	Negative	-0.3371	Positive	0.29