Molecular Profiling of Spermatozoa Reveals Correlations between Morphology and Gene Expression: A Novel Biomarker Panel for Male Infertility

Table 1

Functional classification of the methylated genes using Genomatix software.

Functional categories	GO term ID	value	List of genes
Calcium channel activity	GO:0005262		CACNA1H, CACNA1C, CACNA2D4, TRPM3, JPH3
Dolichyl-phosphate-mannose-protein mannosyltransferase activity	GO:0004169		TMTC4, POMT2
Enzyme binding	GO:0019899		SLC9A3R2, ATP6V0A4, EXOC2, FRS2, EZR, CARHSP1, STXBP5, PHACTR1, CNST, RALGPS2, SH3BP4, AURKA, SMYD3, PRKN, SLC2A1, DENND3, RASGEF1A, JAKMIP3, MARCHF6, HDAC4, SYNE1, ARFGEF3, PSD3, MCF2L, GATA4
Coreceptor activity	GO:0015026		GPC6, CD80, RGMA
ATP-dependent microtubule motor activity	GO:1990939		KIF26A, DNAH2, KIF17
Isomerase activity	GO:0016853		TXNDC5, NAXD, QSOX1, TOP1MT, PDIA6
Ubiquitin-specific protease binding	GO:1990381		PRKN, MARCHF6
Tubulin binding	GO:0015631		KIF26A, EZR, CCDC88B, TBCD, PRKN, JAKMIP3, KIF17
Oxidoreductase activity, acting on a sulfur group of donors	GO:0016667		QSOX1, PDIA6, NXN
ARF guanyl-nucleotide exchange factor activity	GO:0005086		ARFGEF3, PSD3
GTPase binding	GO:0051020		EXOC2, STXBP5, RALGPS2, SH3BP4, DENND3, RASGEF1A, ARFGEF3, PSD3, MCF2L
Protein binding	GO:0005515		TRIM2, CACNA1H, SPSB1, KIF26A, SLC9A3R2, ESPNL, AUTS2, TXNDC5, CACNA1C, TCAF2, ZFYVE28, SPON2, ATP6V0A4, MAD1L1, EXOC2, FRS2, DPF3, ERGIC1, SPATA18, CUX1, EZR, COLEC11, RPA3, GPC6, AHRR, MFAP3L, CARHSP1, DNAH2, ZNRF4, PFKP, BANP, CCDC88B, SCN8A, STXBP5, PHACTR1, CNST, RALGPS2, NAXD, TBCD, PRKG1, JMJD1C, USP10, LRTM2, ST8SIA5, CCDC60, DLGAP2, SH3BP4, AURKA, TG, LTBP2, SMYD3, PRKN, COL4A2, SLC2A1, IGHMBP2, DENND3, SDHA, HMGB4, CHFR, MPHOSPH10, SNTG2, RASGEF1A, JAKMIP3, PDIA6, PHLDB2, MARCHF6, FRK, HDAC4, TBL3, TLE1, SYNE1, ARFGEF3, PSD3, KIF17, ZNF239, FOXP4, MCF2L, FSTL4, ANO2, SOX6, GATA4, AGPAT4, CD80, SPPL2B, TERT, DAB1, RGMA
Passive transmembrane transporter activity	GO:0022803		CACNA1H, CACNA1C, CLCN7, CACNA2D4, SCN8A, TRPM3, JPH3, ANO2
Cytoskeletal protein binding	GO:0008092		KIF26A, ESPNL, CACNA1C, EZR, CCDC88B, STXBP5, PHACTR1, TBCD, PRKN, SNTG2, JAKMIP3, SYNE1, KIF17