miR-149 Suppresses the Proliferation and Metastasis of Human Gastric Cancer Cells by Targeting FOXC1

Figure 2

miR-149 overexpression repressed the proliferation and metastasis of gastric cancer cells. AGS and MKN28 cells were transfected with miR-149 overexpression plasmid or miR-ctrl plasmid. (a) miR-149 expression was examined by qRT-PCR assay after transfection. (b) Cell proliferation was detected using the MTT assay at 24, 48, and 72 h. (c) Colony formation was assessed in AGS/MKN28 cells after transfection. (d) Flow cytometry analysis of cell cycle in AGS and MKN28 cells. (e) Flow cytometry analysis of apoptosis after transfection with miR-149 overexpression plasmid or miR-ctrl plasmid. (f, g) Wound-healing and transwell assays showed that miR-149 suppressed GC cell migration and invasion abilities. (h) Western blot to detect the expression of Bax, Bcl2, CDK4, MMP2, MMP9, and E-cadherin after transfection with miR-149 overexpression plasmid or miR-ctrl plasmid in AGS and MKN28 cells. The difference between the two groups was evaluated by Student’s -test, and data are expressed as . For all data, means , and means . Differences between groups were evaluated using Student’s -test, and data are expressed as . For all data, indicates , and indicates .

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