miR-149 Suppresses the Proliferation and Metastasis of Human Gastric Cancer Cells by Targeting FOXC1

Figure 3

FOXC1 is a direct target of miR-149. (a) miR-149 has the binding sites of the 3-UTR of FOXC1. (b) Luciferase activity in the pmiRGLO-FOXC1-wt group showed a statistically significant decrease following overexpression of miR-149. (c, d) The mRNA and protein expression levels of FOXC1 were detected using qRT-PCR and western blot after transfection with miR-149 overexpression plasmid or miR-ctrl plasmid in AGS and MKN28 cells. Differences between groups were evaluated using Student’s -test, and data are expressed as . For all data, indicates , and indicates .

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