miR-149 Suppresses the Proliferation and Metastasis of Human Gastric Cancer Cells by Targeting FOXC1

Figure 5

Silencing FOXC1 suppresses cell proliferation and metastasis of gastric cancer cells. AGS cells and MKN28 cells were transfected with si-FOXC1-1, si-FOXC1-2, or a negative control. (a) qRT-PCR and western blot were performed to determine the expression of FOXC1 after transfection. (b) Cell proliferation was detected using the MTT assay after transfection. (c) Colony formation was assessed in AGS/MKN28 cells after transfection. (d) Flow cytometry analysis of cell cycle in AGS and MKN28 cells. (e) Flow cytometry analysis of apoptosis after transfection with si-FOXC1-1, si-FOXC1-2, or the negative control. (f, g) Wound-healing and transwell analysis representing the migration and metastatic capacity of AGS and MKN28 cells. (h) Western blot to detect the expression of Bax, Bcl2, CDK4, MMP2, MMP9, and E-cadherin after transfection with si-FOXC1-1, si-FOXC1-2, or a negative control. Differences between groups were evaluated using Student’s -test, and data are expressed as . For all data, indicates , and indicates .

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