miR-149 Suppresses the Proliferation and Metastasis of Human Gastric Cancer Cells by Targeting FOXC1

Table 1

Primers and oligonucleotides used in this work.

Name	Sequence (5–3)
miR-149-RT	GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAA; TTGCACTGG ATACGACGGGAGTG
miR-149-F	ATCCAGTGCGTGTCGTG
miR-149-R	TGCTTCTGGCTCCGTGTCTT
U6-RT	CGCTTCACGAATTTGCGTGTCAT
U6- F	GCTTCGGCAGCACATATACTAAAAT
U6-R	CGCTTCACGAATTTGCGTGTCAT
FOXC1-F	TGTTCGAGTCACAGAGGATCG
FOXC1-R	ACAGTCGTAGACGAAAGCTCC
GAPDH-F	GTAGAGGCAGGGATGATGTTC
GAPDH-R	GCCAAAAGGGTCATCATCTC
Pre-miR-149	GCCGGCGCCCGAGCUCUGGCUCCGUGUCUUC; ACUCCCGUGCUUGUCCGAGGAGGGAGGGAGGG; ACGGGGGCUGUGCUGGGGCAGCUGGA
FOXC1-3UTR	AUUAUACAUCCCUGUGAGCCAGA
miR-149-sense	AATTCGCCGGCGCCCGAGCTCTGGCTCCGTGTCTT; CACTCCCGTGCTTGTCCGAGGAGGGAGGGAGGGA; CGGGGGCTGTGCTGGGGCAGCTGGAA
miR-149-antisense	AGCTTTCCAGCTGCCCCAGCACAGCCCCCGTCCCTC; CCTCCCTCCTCGGACAAGCACGGGAGTGAAGACACG; GAGCCAGAGCTCGGGCGCCGGCG
FOXC1-3UTR-WT	CATTATACATCCCTGTGAGCCAGAC
FOXC1-3UTR-WB	TCGAGTCTGGCTCACAGGGATGTATAATGAGCT
FOXC1-3UTR-MT	CATTATACATCCCTGTATCGGTAAC
FOXC1-3UTR-MB	TCGAGTTACCGATACAGGGATGTATAATGAGCT
NC-sense	UUCUCCGAACGUGUCACGUTT
NC-antisense	ACGUGACACGUUCGGAGAATT
si-FOXC1-1-sense	CGGGAAUAGUAGCUGUCAATT
si-FOXC1-1-antisense	UUGACAGCUACUAUUCCCGTT
si-FOXC1-2-sense	ACAAGAAGAUCACCCUGAATT
si-FOXC1-2-antisense	UUCAGGGUGAUCUUCUUGUTT