Variant Analysis of Alkaptonuria Families with Significant Founder Effect in Jordan

Table 1

Primer oligonucleotide sequences used in Sanger sequencing.

Exon	Primer sequence (5-3)	Amplicon size (bp)	Annealing temperature (°C)
1	F: GAGTTAGACAATTCTTTCAGC	418	51
	R: ATGAACAAAGGCAAGGGATG
2	F: GCAATATCCAGCACTCTTCTGA	437	55
	R: CCCCTATGACTTGGGAAACC
3	F: GGGGCAAGTCACATCAAAAG	415	53
	R: GCTGGCAGGAAGTTCATTCT
4	F: TTGGCAGCATGGAAATAACC	516	54
	R: TTTGAGCAGAAAACAGACACACT
5	F: AGCATGAAAAGCAGCATCAG	560	53
	R: ACGCAGGTGGTTTTGTCTCT
6	F: GTCAGTAAATTCAGGCTCCTTAGA	521	57
	R: TCCATCCTCCCTTTTCTGTTT
7	F: CGCTATTCTTTCATTCCCTCA	530	52
	R: GTCCAGAAGAGATGGGCAAA
8	F: ACAAGTTCCTTGCCTGGTGA	439	53
	R: CTCAGATTCCCTCCTCGTTG
9	F: CCAAGCAGCTCAACAAACAA	319	55
	R: AGTGAGACAGCGAAGGGAGA
10	F: CTCTCTTCCCTTCCCCTCAC	551	57
	R: TTTGTAGTGCCGTAGTGGTATGA
11	F: TCTCCCAAAGGACGGTAAAA	391	53
	R: CTCCCTCACCAAAGGACAAA
12	F: CAGATCCCTACCCCAAACCT	600	56
	R: CACGAGCCAAATGAACCTCT
13	F: TGCCAAGAATGCCAATATGA	478	60
	R: CCCTCTTTTGACTCTTCCTCTG
14	F: ACCAGAGCCACAACTCAGG	576	55
	R: CTGCCAGGTTTGTCTCATCA