Anisakis pegreffii Extract Induces Airway Inflammation with Airway Remodeling in a Murine Model System

Figure 7

Expression of the IL-4⁺ CD4⁺ marker in lung lymph nodes (LLN). Expression of the IL-4 marker in the LLN of Anisakis pegreffii extract- (AE-) sensitized and control mice was analyzed using flow cytometry. Lymphocytes from LLN were incubated with a stimulated anti-CD3e antibody. After staining, lymphocytes were initially gated for CD4⁺ cells, and the percentage of IL-4⁺ cells was calculated using FACS analysis. The IL-4⁺ CD4⁺ T cell number is plotted in the right panel.