Establishment and Validation of the Detection of TERT Promoter Mutations by Human Gliomas U251 Cell Lines

Table 2

Precision of the Sanger sequencing assay for TERTp mutations. Performance of interassay and intra-assay coefficients of variation.

Concentration		Total			Within-day			Between-day
		Mean	SD	CV (%)	Mean	SD	CV (%)	Mean	SD	CV (%)
50%	30	1.14	0.12	10.72	1.21	0.02	1.84	1.20	0.12	10.00
15%	30	7.14	0.93	13.02	5.67	0.13	2.26	6.61	0.71	10.73

TERTp: the promoter region of the telomerase reverse transcriptase gene; SD: standard deviation; CV: coefficient of variation.