Novel Elongator Protein 2 Inhibitors Mitigating Tumor Necrosis Factor-α Induced Osteogenic Differentiation Inhibition

Figure 3

The ALP stain and activity measurements of 2# and 5# candidate on C2C12 and MC3T3-E1 cell osteogenic differentiations. 2# and 5# compounds show remarkable mitigation against TNF-α induced ALP activity inhibition. The ALP staining observation in each group (a, c). The relative ALP activity were measured from the cell lysates of each group (b, d). Data are ; , , each experiment group vs. the TNF-α treatment cell group.

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