Dexmedetomidine Directs T Helper Cells toward Th1 Cell Differentiation via the STAT1-T-Bet Pathway

Figure 2

Dexmedetomidine enhances IFN-γ production by CD4+ T cells. After CD4+ T cells were incubated with anti-CD3/CD28 and rIL-2 for 3 days, different concentrations of dexmedetomidine (0, 0.01, 0.1, 1, and 10 nM) were administered for another 24 hours. IFN-γ and IL-4 production (a) were measured by ELISA. IFN-γ and IL-4 (b) mRNA expression was tested by real-time PCR as described in Methods and Materials. The data are representative of at least three independent experiments with consistent results. Student’s -test was performed to detect between-group differences. The results shown are the . , .

(a)

(b)