Dexmedetomidine Directs T Helper Cells toward Th1 Cell Differentiation via the STAT1-T-Bet Pathway

Figure 4

Atipamezole inhibits dexmedetomidine-induced Th1 cell differentiation and cytokine production. After being stimulated by anti-CD3/CD28 and rIL-2 for 3 days, CD4+ T cells were cultured in the presence or absence of dexmedetomidine (0.1 nM) and/or atipamezole (1 nM) for 24 hours. Th1 and Th2 cell concentrations (a) were measured by flow cytometry. The production (b) and expression (c) of related cytokines were detected by ELISA and real-time PCR, respectively. The data are representative of at least three independent experiments with consistent results. Student’s -test was performed to detect between-group differences. The results shown are the . , .

(a)

(b)

(c)