Dexmedetomidine Directs T Helper Cells toward Th1 Cell Differentiation via the STAT1-T-Bet Pathway

Figure 7

Anti-STAT1 (fludarabine) reverses dexmedetomidine-induced STAT1 phosphorylation and T-bet expression. After stimulation by anti-CD3/CD28 and rIL-2 for 3 days, CD4+ T cells were cultured in the presence or absence of dexmedetomidine (0.1 nM) and/or fludarabine (100 nM) for 24 hours. T-bet and GATA3 (a) mRNA expressions were determined by real-time PCR. STAT1 phosphorylation and T-bet expression (b) were defined using western blots. The data are representative of at least three independent experiments with consistent results. Student’s -test was performed to detect between-group differences. The results shown are the . , .

(a)

(b)