Full Genomic Sequences of H5N1 Highly Pathogenic Avian Influenza Virus in Human Autopsy Specimens Reveal Genetic Variability and Adaptive Changes for Growth in MDCK Cell Cultures

Table 2

Nucleotide heterogeneities of HPAI H5N1 viral genomes in autopsy specimens as demonstrated by NGS.

Segment	Nucleotide position^1	Type of autopsy specimens				Amino acid change
		Trachea	Lung	Feces	Colon
PB2	945	G	A	A	A	M315I
PB1	549	A (63%); G (37%)	A (61%); G (39%)	A (51%); G (49%)	G (52%); A (48%)	—
PA	1882	A	G	G	G	M628V
HA	1230	G	A (62%); G (38%)	G	G	—
NP	449	A (53%); G (47%)	G	G	G	K150R
	605	G (59%); A (41%)	A	A	A	S202N
NA	399 (459)^2	T	C	C	T	—
	706 (763)^2	T	G (53%); T (47%)	T	T	V255F
	723 (780)^2	A	A	G	A	—

Note: ¹nucleotide numbering based on influenza A/Thailand/1(KAN-1)/2004 (H5N1). ²NA numbering based on N2 subtype.