miR-296-5p Inhibits the Secretion of Pulmonary Surfactants in Pulmonary Epithelial Cells via the Downregulation of Wnt7b/β-Catenin Signaling

Figure 2

Lentiviral vector-mediated transfection was used to establish stable overexpression of miR-296-5p in A549 cell lines. (a). The transfection efficiency of lentivirus mir-296-5p in A549 cells was detected using fluorescence. (b). The RT-qPCR assay was used to detect miR-296-5p expression after transfection. .

(a)

(b)