Overexpression of Limb Bud and Heart Alleviates Sepsis-Induced Acute Lung Injury via Inhibiting the NLRP3 Inflammasome

Figure 2

LBH increased the viability and attenuated inflammation of lipopolysaccharide- (LPS-) induced A549 cells. (a) The transfection efficiency of sh-NC, sh-LBH, pcDNA-NC (pcDNA3.1), and pcDNA3.1-LBH (oe-LBH) was demonstrated by using qRT-PCR. vs. sh-NC, ^### vs. pcDNA3.1. (b) The viability of LPS-induced A549 cells was measured by MTT assay. vs. sh-NC, ^## vs. pcDNA3.1. (c) qRT-PCR was performed to confirm the expression level of IL-1β, IL-6, and IL-18 in LPS-induced A549 cells. and vs. sh-NC, ^# and ^## vs. pcDNA3.1.

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