In Vitro Anticancer Activity of Imperata cylindrica Root’s Extract toward Human Cervical Cancer and Identification of Potential Bioactive Compounds

Figure 3

(a) Apoptotic effect of IC-MeOH in CaSki cells. Untreated CaSki cells (control) and treated CaSki cell population with IC-MeOH or cisplatin (1/2 IC₅₀, IC₅₀, and 2IC₅₀) at different stages of apoptosis after 24 h and 48 h. Data from 10.000 cells had been collected per data file; in four zones of the drawn quadrant, we had viable cells (Q1), cells bounded to annexin V-PE only (Q2: early apoptotic cells), and cells bounded to both annexin V-PE and 7-AAD (Q3: late apoptotic cells). IC-MeOH: Imperata cylindrica root’s methanol extract; IC₅₀: inhibitory concentration 50 of IC-MeOH; ½ IC₅₀: half of the inhibitory concentration 50 of IC-MeOH; 2 IC₅₀: two times the inhibitory concentration 50 of IC-MeOH; 24 h, 48 h, and 72 h: treatments’ times. (b) Percentage of apoptotic cells (early and late) in the control (untreated CaSki cells) and treated CaSki cells. All data presented are the and are representative of three independent experiments. values < 0.05 were considered to be statistically significant, and significance was marked as p values < 0.05, values < 0.01, and values < 0.001. IC-MeOH: Imperata cylindrica root’s methanol extract; Cntrl: control; IC₅₀: inhibitory concentration 50 of IC-MeOH; 1/2 IC₅₀: half of the inhibitory concentration 50 of IC-MeOH; 2 IC₅₀: two times the inhibitory concentration 50 of IC-MeOH; CaSki_24 h: CaSki cells treated during 24 h; CaSki_48 h: CaSki cells treated during 48 h.

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