IFN-γ Licensing Does Not Enhance the Reduced Immunomodulatory Potential and Migratory Ability of Differentiation-Induced Porcine Bone Marrow-Derived Mesenchymal Stem Cells in an In Vitro Xenogeneic Application

Figure 1

Characterization of porcine BMMSCs with or without IFN-γ licensing. (a) Morphology of undifferentiated BMMSCs with or without IFN-γ licensing (bright fields) and cytochemical observation of differentiated BMMSCs toward adipocytes (Oil red O) and osteoblasts (Von kossa and alizarin red) with or without IFN-γ licensing. Magnification: ×100. Scale bar: 100 μm. (b) The expression of lineage-specific genes after differentiation toward adipocytes (PPAR-γ and FABP-4) and osteoblasts (ON and OPN). Data represent the . and axes show types of cells and the relative expression of each gene, respectively. indicated significant difference from BMMSCs-IFN-γ. ^# indicated significant difference between each differentiated MSCs and their IFN-γ-licensed cells (ADI-IFN-γ vs. ADI+IFN-γ or OST-IFN-γ vs. OST+IFN-γ). ^§ indicated significant difference between BMMSCs+IFN-γ and IFN-γ-licensed differentiated cells (BMMSCs+IFN-γ vs. ADI+IFN-γ or BMMSCs+IFN-γ vs. OST+IFN-γ). Abbreviations: BMMSCs: porcine bone marrow-derived mesenchymal stem cells; IFN-γ: interferon γ; BMMSCs-IFN-γ or BMMSCs+IFN-γ: undifferentiated BMMSCs without or with 50 ng/mL human IFN-γ supplementation during 48 h, respectively; ADI-IFN-γ or ADI+IFN-γ: differentiated BMMSCs toward adipocytes without or with 50 ng/mL human IFN-γ supplementation during 48 h, respectively; OST-IFN-γ or OST+IFN-γ: differentiated BMMSCs toward osteoblasts without or with 50 ng/mL human IFN-γ supplementation during 48 h, respectively; PPAR-γ: peroxisome proliferator-activated receptor gamma; FABP-4: fatty acid-binding protein 4; ON: osteonectin; OPN: osteopontin.

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