IFN-γ Licensing Does Not Enhance the Reduced Immunomodulatory Potential and Migratory Ability of Differentiation-Induced Porcine Bone Marrow-Derived Mesenchymal Stem Cells in an In Vitro Xenogeneic Application

Figure 2

Assessment of MSC-specific surface molecule expressions of undifferentiated and differentiated MSCs with or without IFN-γ licensing. (a) Representative images of histograms for MSC-specific (CD44, CD90, and CD105) and hematopoietic (CD45) surface molecules detected by flow cytometry. (b) Statistical assessment of surface molecule expression in six groups (BMMSCs-IFN-γ, BMMSCs+IFN-γ, ADI-IFN-γ, ADI+IFN-γ, OST-IFN-γ, and OST+IFN-γ). Data represent the . and axes show types of cells and positive cell population (%), respectively. indicated significant difference from BMMSCs-IFN-γ. ^§ indicated significant difference between BMMSCs+IFN-γ and IFN-γ-licensed differentiated cells (BMMSCs+IFN-γ vs. ADI+IFN-γ or BMMSCs+IFN-γ vs. OST+IFN-γ). A significant difference between each differentiated MSCs and their IFN-γ-licensed cells was not found (; ADI-IFN-γ vs. ADI+IFN-γ or OST-IFN-γ vs. OST+IFN-γ.

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