IFN-γ Licensing Does Not Enhance the Reduced Immunomodulatory Potential and Migratory Ability of Differentiation-Induced Porcine Bone Marrow-Derived Mesenchymal Stem Cells in an In Vitro Xenogeneic Application

Figure 3

Effect of IFN-γ licensing in expression of immunosuppressive molecules in undifferentiated and differentiated MSCs with or without IFN-γ licensing. (a) IDO activity measurements using the level of L-kynurenine derived from six groups. Data represent the . and axes presented types of cells and the concentration of L-kynurenine (μM), respectively. (b) Expression of immunosuppressive molecules (IDO, TSG-6, IL-10, and TGF-β1) in the six groups by qPCR. Data represent the . and axes represent types of cells and the relative expression of each gene, respectively. indicated significant difference from BMMSCs-IFN-γ. ^# indicated significant difference between each differentiated MSCs and their IFN-γ-licensed cells (ADI-IFN-γ vs. ADI+IFN-γ or OST-IFN-γ vs. OST+IFN-γ). ^§ indicated significant difference between BMMSCs+IFN-γ and IFN-γ-licensed differentiated cells (BMMSCs+IFN-γ vs. ADI+IFN-γ or BMMSCs+IFN-γ vs. OST+IFN-γ). Abbreviations: IDO: indoleamine 2,3 deoxygenase; TSG-6: tumor necrosis factor-stimulated gene 6; IL-10: interleukin 10; TGF-β1: transforming growth factor beta 1; qPCR: quantitative polymerase chain reaction.

(a)

(b)