IFN-γ Licensing Does Not Enhance the Reduced Immunomodulatory Potential and Migratory Ability of Differentiation-Induced Porcine Bone Marrow-Derived Mesenchymal Stem Cells in an In Vitro Xenogeneic Application

Figure 4

Alteration of the immunomodulatory potential of undifferentiated and differentiated MSCs with or without IFN-γ licensing in human PBMCs as a xenogeneic application by MLR and ELISA assay. (a) MLR assay with mitomycin C-treated MSCs for each of six groups and for PHA-stimulated human PBMCs, resulting in an MSC : PBMC ratio of 1 : 10. Data represent the . and axes represent types of cells and the proliferated PBMCs (%), respectively; PHA-activated PBMCs seeded alone were counted as 100%. (b) An ELISA assay was used to detect the concentration of TNF-α in the supernatant post-MLR assay. Data represent the . and axes present types of cells and the concentration of TNF-α (pg/mL), respectively. indicated significant difference from BMMSCs-IFN-γ. ^§ indicated significant difference between BMMSCs+IFN-γ and IFN-γ-licensed differentiated cells (BMMSCs+IFN-γ vs. ADI+IFN-γ or BMMSCs+IFN-γ vs. OST+IFN-γ). A significant difference between each differentiated MSCs and their IFN-γ-licensed cells was not found (; ADI-IFN-γ vs. ADI+IFN-γ or OST-IFN-γ vs. OST+IFN-γ). Abbreviations: PBMCs: peripheral blood mononuclear cells; MLR: mixed lymphocyte reaction; ELISA: enzyme-linked immunosorbent assay; PHA: phytohemagglutinin; TNF-α: tumor necrosis factor α.

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