IFN-γ Licensing Does Not Enhance the Reduced Immunomodulatory Potential and Migratory Ability of Differentiation-Induced Porcine Bone Marrow-Derived Mesenchymal Stem Cells in an In Vitro Xenogeneic Application

Figure 5

Change in the migratory ability of undifferentiated and differentiated MSCs with or without IFN-γ licensing, as detected with a scratch assay and gene expression analysis. (a) Representative images of the scratch assay for the six groups. After inducing an initial scratch at time point 0 h, MSCs were allowed to migrate to the scratched area for 48 h. Magnification: ×100. Scale bar: 100 μm. (b) Statistical assessment of migratory ability of the six tested groups. The migrated areas postscratch were normalized to the value obtained from BMMSCs-IFN-γ at the 48 h time point. Data represent the . and axes present types of cells and the relative migrated area, respectively. (c) Gene expression of migration-related genes (ICAM-1 and VCAM-1) in the six groups, as detected by qPCR. Data represent the . and axes presented types of cells and the relative expression of each gene, respectively. indicated significant difference from BMMSCs-IFN-γ. ^§ indicated significant difference between BMMSCs+IFN-γ and IFN-γ-licensed differentiated cells (BMMSCs+IFN-γ vs. ADI+IFN-γ or BMMSCs+IFN-γ vs. OST+IFN-γ). A significant difference between each differentiated MSCs and their IFN-γ-licensed cells was not found (; ADI-IFN-γ vs. ADI+IFN-γ or OST-IFN-γ vs. OST+IFN-γ). Abbreviations: ICAM-1: intercellular adhesion molecule 1; VCAM-1: vascular cell adhesion molecule 1.

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