IFN-γ Licensing Does Not Enhance the Reduced Immunomodulatory Potential and Migratory Ability of Differentiation-Induced Porcine Bone Marrow-Derived Mesenchymal Stem Cells in an In Vitro Xenogeneic Application

Figure 6

Changes in the migratory ability of undifferentiated and differentiated MSCs with or without IFN-γ licensing, as detected by a Transwell assay with human PBMCs as a xenogeneic application. (a) Representative images from the Transwell assay with MSCs from six tested groups in the upper chamber toward PHA-stimulated human PBMCs in the lower chamber. Crystal violet-stained cells indicate MSCs that migrated through membranes during 1 day. Magnification: ×100. Scale bar: 100 μm. (b) Statistical assessment of the migratory ability of the six groups. The population of migrating MSCs was normalized to the value obtained from BMMSCs-IFN-γ. Data represent the . and axes represent types of cells and the relative population of migrating cells, respectively. indicated significant difference from BMMSCs-IFN-γ. ^§ indicated significant difference between BMMSCs+IFN-γ and IFN-γ-licensed differentiated cells (BMMSCs+IFN-γ vs. ADI+IFN-γ or BMMSCs+IFN-γ vs. OST+IFN-γ). A significant difference between each differentiated MSCs and their IFN-γ-licensed cells was not found (; ADI-IFN-γ vs. ADI+IFN-γ or OST-IFN-γ vs. OST+IFN-γ).

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