lncRNA OXCT1-AS1 Promotes Metastasis in Non-Small-Cell Lung Cancer by Stabilizing LEF1, In Vitro and In Vivo

Figure 6

LEF1 knockdown inhibits the migration and invasion of NSCLC cells. (a, b) LEF1 expression in LEF1 stably depleted (sh-LEF1) and negative control (sh-NC) H1299 and A549 cells was assessed by western blot. (c, d) Cell proliferation assays for H1299 (c) and A549 cells (d) transfected with shRNA (sh-LEF1) or negative control (sh-NC) were measured using CCK-8 assays. (e, f) Transwell assays were performed to determine the migration of H1299 and A549 cells. Quantitative data were from 5 fields; . (g, h) Transwell assays were used to assess the invasion of H1299 and A549 cells. Quantitative data were from 5 fields; . (i–l) Western blot was performed to measure the level of E-cadherin, N-cadherin, vimentin, and Snail in H1299 and A549 cells, as normalized to the β-actin level.

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