Recurrent Superenhancer of the Oncogene POU5F1B in Colorectal Cancers

Figure 2

HCT116 superenhancers overlapped with robust CRC genes at POU5F1B. (a) Venn diagram of robust CRC DEGs (differentially expressed genes) and the genes associated with the top 20 SEs (superenhancers) in HCT116; (b, c) COLO205 Chip-qPCR of anti-H3K27ac and BRD4 of POU5F1B with or without JQ-1 treatment; (d) COLO205 qPCR of POU5F1B with or without JQ-1 treatment; (e) Western blot siRNA knocking down effect obvious in the protein level; (f) transwell assay showing that migration of the tumor cells was reduced after POU5F1B downregulation; (g) the high POU5F1B expression group had worse overall survival.

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