[Retracted] Mangifera indica Extracts as Novel PKM2 Inhibitors for Treatment of Triple Negative Breast Cancer

Figure 1

Protein expression, purification, and establishment of enzymatic activity assay. (a) Double enzyme digestion for checking of insert. (b) Purity check of the purified recombinant PKM2 protein. (c) Principle of PKM2 enzymatic activity assay. (d) Optimization of substrate concentration (PEP) for PKM2 enzymatic assay.

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