β-Sitosterol Alters the Inflammatory Response in CLP Rat Model of Sepsis by Modulation of NFκB Signaling

Table 1

Reference and target genes and the corresponding primers used in RT-qPCR.

Symbol	Gene name	Mechanism	GenBank ID	Primer sequences	Amplicon length (bp)
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	Glycolytic enzyme	NM 017008	FP: 5-AGTGCCAGCCTCGTCTCATA-3; RP: 5-GGTAACCAGGCGTCCGATA-3	77
TNF-α	Tumor necrosis factor-alpha	Proinflammatory cytokine	NM 012675	FP: 5-TGGGCTCCCTCTCATCAGTT-3; RP: 5-CTTGGTGGTTTGCTACGACG-3	104
NF-κBi	Nuclear factor kappa B inhibitor-alpha	Inhibitory enzyme	NM001105720	FP: 5-GTGACTTTGGGTGCTGATGT-3; RP: 5-ACACTTCAACAGGAGCGAGA-3	111

bp: base pair; FP: forward primer; RP: reverse primer.