Attenuation in Proinflammatory Factors and Reduction in Neuronal Cell Apoptosis and Cerebral Vasospasm by Minocycline during Early Phase after Subarachnoid Hemorrhage in the Rat

Figure 3

Immunohistochemistry staining of TUNEL assay and western blot analysis to show the effect of minocycline or fluorocitrate treatment on apoptosis in the cortex of rats subjected to SAH. (a) The region for immunohistochemistry staining of TUNEL assay. (b) Immunohistochemistry staining of TUNEL assay for minocycline (50 mg/kg) or fluorocitrate (50 mg/kg) was injected intraperitoneally three days prior to the induction of SAH, and the cortex tissues were collected at 6 hr postsurgery. (c) Western blot assay of cleaved caspase-3 for minocycline (50 mg/kg) or fluorocitrate (50 mg/kg) was injected intraperitoneally three days before surgery. (d) The cleaved caspase-3 expression levels were normalized sham group. compared with the sham group. ^### compared with the SAH+minocycline group.

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