A Possible Flow Cytometry-Based Viability and Vitality Assessment Protocol for Pathogenic Vibrio cholerae O1 and O139 Postexposure to Simulated Gastric Fluid

Figure 2

Flow cytometry multiparameter dot plots representing Vibrio cholerae O1 bacterial control cell suspensions stained with fluorescent probes: TO, CFDA-AM, and PI and analysed using a BD C6 Accuri flow cytometry system with CSampler™. The -axis indicates the logarithmic green fluorescence intensity of CFDA-AM and TO (FL1). The -axis indicates the logarithmic red fluorescence intensity of PI (FL3). Untreated bacterial controls include pure culture samples (b, c, d, g, h, and i) and dead control culture samples (d, e, i, and j). Treated simulated gastric fluid treated bacteria are represented on (f, k). Specific bacterial subpopulations are distinguished by scatter plots: gating of bacterial population on R1 (a) background noise/debris, unstained (black) cells (b, g) intact (green) population labelled with TO (c), or CFDA-AM (h). Mixed/stressed (blue) population labelled with TO+PI (d, f) or CFDA-AM+PI (i, k), and damaged (red) population labelled with PI (e, j).

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