Mitophagy Impairment Aggravates Cisplatin-Induced Ototoxicity

Figure 2

Knockdown of Parkin and BNIP3 enhances the reduction in HEI-OC1 cell viability following cisplatin (CP) treatment and reduces the expression of mitophagy-associated proteins. (a) The survival rates of HEI-OC1 cells in the si-Parkin and si-BNIP3 transfected groups relative to that in si-Control transfected and cisplatin alone treatment groups. (b) Representative immunoblots of cell lysates harvested after the indicated treatments showing PINK1, Parkin, BNIP3, LC3B, and COX IV (loading control) expression. (c) Relative expression levels of PINK1, Parkin, BNIP3, and LC3B were increased post cisplatin treatment alone but significantly downregulated upon Parkin and BNIP3 siRNA transfection prior to cisplatin treatment. Data presented as the of five independent experiments. ; .

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