Mitophagy Impairment Aggravates Cisplatin-Induced Ototoxicity

<div>Cisplatin (CP) enhances the rate of mitophagy in HEI-OC1 cells while the knockdown of <i>Parkin</i> and <i>BNIP3</i> expression reverses this effect. Mitophagosomes and mitophagolysosomes were detected by colocalization (yellow puncta) of LC3B (green) with MitoTracker (red) (a, upper), and LAMP1 (green) with MitoTracker (red) (b, upper), respectively. The area of overlap in HEI-OC1 cells transfected with si-Control was enhanced by cisplatin treatment (10 <i>μ</i>M, 24 h), while colocalization areas were significantly reduced in cells transfected with <i>Parkin</i> and <i>BNIP3</i> siRNAs prior to cisplatin treatment. 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BioMed Research International

Mitophagy Impairment Aggravates Cisplatin-Induced Ototoxicity

Figure 3