Factors Associated with Promoted Proliferation of Osteosarcoma by Peptidylarginine Deiminase 4

Figure 1

PADI4 upregulates proliferation and colony formation of U2OS and Saos-2 cells. (a, e) U2OS cells (a) and Saos-2 cells (e) were treated with PADI4 inhibitor Cl-amidine, and cell viability was detected by CCK8 assay. (b, f) Cell viability was detected by CCK8 assay after overexpression of PADI4 in U2OS (b) and Saos-2 cells (f). (c, g) The colony forming capacity was measured in U2OS (c) and Saos-2 cells (g) after treatment with Cl-amidine or being transfected with PADI4 constructs. (d, h) Analyze and calculate the colony density by ImageJ. Data are presented as the of three independent experiments ().

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