Antitumor Effect of Saikosaponin A on Human Neuroblastoma Cells

Figure 2

The morphological changes of human neuroblastoma cells and cell nucleus after treatment with SSA. (a) Morphological changes of human NB cells SK-N-AS treated with different concentrations of SSA (0, 12.5, 15, and 17.5 μM) for 24 h or 48 h under a light microscope (100x). (b) Cell nuclear morphological changes of human NB cells SK-N-AS treated with SSA by Hoechst 33258 staining in the same field observed under a light and fluorescent microscope (400x). Nuclei indicated by the arrows were round, irregular, condensed, and fragmented by treatment with SSA at 0 μM, 12.5 μM, 15 μM, and 17.5 μM, respectively.

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