Cilengitide Inhibits Neovascularization in a Rabbit Abdominal Aortic Plaque Model by Impairing the VEGF Signaling

Figure 8

The effect of cilengitide on the proliferation, migration, apoptosis, and tube formation of HUVECs. (a) The quantification of cell proliferation was evaluated by CCK-8 assay for HUVECs under treatment with different concentrations of cilengitide (0-10 μM) for 24 hours. (b, c) The representative image and quantification of cell migration evaluation of HUVECs under treatment with different concentrations of cilengitide (0-10 μM) for 24 hours. Scale bar, 100 μm. (d, e) The representative image and quantification of apoptosis were evaluated by flow cytometry analysis for HUVECs under treatment with different concentrations of cilengitide (0-10 μM) for 24 hours. (f) The representative image of tube formation assay of HUVECs under treatment with different concentrations of cilengitide (0-10 μM) for 8 hours. (g–i) Quantification of the length of tubes, tube area, and the number of capillary-like structures for the tube formation assay of HUVECs. for each group. Scale bar, 100 μm. vs. 0 μM; vs. 0 μM.

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