[Retracted] Cannabinoid WIN 55,212-2 Inhibits Human Glioma Cell Growth by Triggering ROS-Mediated Signal Pathways

Figure 5

WIN triggers ROS-mediated DNA damage in U251 cells. (a) WIN caused time-dependent ROS generation in U251 cells. U251 cells seeded in 6-well plate were treated with 20 μM WIN for 0-120 min and loaded with DCFH-DA probe for 20 min in darkness. Then, cells were washed, and the ROS generation was imaged by a fluorescence microscope (magnification, ×200). (b) Quantitative analysis of ROS level. (c) GSH level. The ELIAS method was used to detect the GSH level in WIN-treated U251 cells. (d) WIN triggered time-dependent DNA damage in U251 cells. (e) ROS inhibition inhibited WIN-induced DNA damage in U251 cells. Cells were pretreated with GSH for 2 h and cotreated with 20 μM WIN for 48 h. Protein expression was detected by western blotting assay. All experiments were repeated three times.

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