Characterization of the Modulatory Effect of Hydroxychloroquine on ACE2 Activity: New Insights in relation to COVID-19

<div>Modulation of ACE2 activity by SARS-CoV-2 spike glycoprotein and its interaction with the inhibitors MLN-4760 and HCQ. Enzyme activity was assayed in the presence of SARS-CoV-2 spike protein (SP), ACE2 inhibitor MLN4760 (MLN), aminoquinoline hydroxychloroquine (HCQ), or their combinations. (a) ACE2 activity in presence of HCQ, MLN4760, or its combination was measured in a kinetic assay using the SensoLyte®390 kit. Fluorescence level and linear regression are represented for each reaction. (b) Kinetic assay showing the effect of SP on HCQ inhibition. (c) Quantification of the effect of SP, MLN, and HCQ on ACE2 activity. SP effect on the inhibition of ACE2 produced by (d) MLN and (e) HCQ. (f) Combined effect HCQ and MLN on ACE2 activity. 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BioMed Research International

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Figure 2

Figure 2: Characterization of the Modulatory Effect of Hydroxychloroquine on ACE2 Activity: New Insights in relation to COVID-19 