A Combination of Geniposide and Chlorogenic Acid Combination Ameliorates Nonalcoholic Steatohepatitis in Mice by Inhibiting Kupffer Cell Activation

Figure 5

GC inhibited the activation of KCs and decreased proinflammatory factor and chemokine levels in the mice liver tissue. F4/80 and TNF-α protein levels in mice were detected by immunoblotting, and the relative expression levels of proteins were corrected by GAPDH (a–c); F4/80 protein expression in mice was detected by immunohistochemical (d). Protein expression of proinflammatory interleukin IL-1α, IL-1β, IL-6, MCP-1, GM-CSF, and TNF-α (e–j). Con: control diet; HFHC: high-fat/carbohydrate/cholesterol/choline diet; GC: geniposide and chlorogenic acid combination. , .

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