Tetrahydrocurcumin Ameliorates Skin Inflammation by Modulating Autophagy in High-Fat Diet-Induced Obese Mice

Figure 2

Body weight, blood glucose levels, and TNF-α mRNA and protein expressions in the regular diet (RD), high-fat diet (HFD), and HFD plus tetrahydrocurcumin supplementation (HFD+T100) groups. (a) After 12 weeks of treatment, the HFD group presented a significantly higher body weight than the RD group, whereas the HFD+T100 group presented a significantly lower body weight than the HFD group. (b) After 12 weeks of treatment, the HFD group presented a significantly higher blood glucose level than the RD group. The HFD+T100 group presented a lower blood glucose level than the HFD group, although the difference was not significant. (c) qRT-PCR analysis of TNF-α expression in the skin of RD (), HFD (), and HFD+T100 () mice. Relative mRNA expression was normalized to GAPDH expression. Fold differences in expression were calculated using the comparative CT method, standardized to the RD value. (d) Western blotting of TNF-α in the skin of RD, HFD, and HFD+T100 mice. Data were normalized to GAPDH expression. Data are representative of three independent experiments. (e) Differences in western blot quantification were determined. Data are presented as of three independent experiments. All comparisons utilized one-way ANOVA with Tukey’s post hoc tests. , , and . RD: regular diet; HFD: high-fat diet; HFD+T100: high-fat diet+tetrahydrocurcumin (100 mg/kg).

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