Tetrahydrocurcumin Ameliorates Skin Inflammation by Modulating Autophagy in High-Fat Diet-Induced Obese Mice

Figure 3

Nox2, Nox4, and Nrf2 expressions in RD, HFD, and HFD+T100 mouse skin. (a–c) qRT-PCR analysis of (a) Nox2, (b) Nox4, and (c) Nrf2 mRNA expressions in the skin of RD (), HFD (), and HFD+T100 () mice. Relative mRNA expression was normalized to GAPDH expression. Fold differences in the expression were calculated using the comparative CT method, standardized against the RD value. (d) Western blotting analysis of Nox2, Nox4, and Nrf2 protein expressions in the skin of RD (), HFD (), and HFD+T100 () mice. Data were normalized to GAPDH expression. The results are representative of three independent experiments. (e–g) Differences in western blot quantification were determined. Data are presented as of three independent experiments. All comparisons utilized one-way ANOVA and Tukey’s post hoc tests. , , and . RD: regular diet; HFD: high-fat diet; HFD+T100: high-fat diet+tetrahydrocurcumin (100 mg/kg).

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