Tetrahydrocurcumin Ameliorates Skin Inflammation by Modulating Autophagy in High-Fat Diet-Induced Obese Mice

Figure 5

p65 expression and phosphorylation in RD, HFD, and HFD+T100 mouse skin. (a) qRT-PCR analysis of p65 expression in RD (), HFD (), and HFD+T100 () mouse skin. Relative mRNA expression, determined by qRT-PCR, was normalized to GAPDH expression. Fold differences in expression were calculated using the comparative CT method, standardized against the RD value. (b) Western blotting of phosphorylated p65 levels in the skin of RD (), HFD (), and HFD+T100 () mice. Data were normalized to p65 and GAPDH expressions. The results are representative of three independent experiments. (c) Differences in western blot quantification were determined. Data are presented as of three independent experiments. All comparisons utilized one-way ANOVA and Tukey’s post hoc tests. and . RD: regular diet; HFD: high-fat diet; HFD+T100: high-fat diet+tetrahydrocurcumin (100 mg/kg); pp65: phosphorylated p65.

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