Downregulation of miR-497-5p Improves Sepsis-Induced Acute Lung Injury by Targeting IL2RB

Figure 4

SP1 regulated miR-497-5p expression via binding to its promoter. (a) The expression of SP1 in the normal group and sepsis group in the database. (b) The dataset was used to predict the binding sites of SP1 in miR-497-5p promoter. (c) qRT-PCR for SP1 and miR-497-5p detection in BEAS-2B cells treated with si-SP1 or pcDNA3.1-SP1. (d) ChIP assay and (e) luciferase activity analyses for the SP1-binding site analysis.

(a)

(b)

(c)

(d)

(e)