Autophagy Is Required for Hepatic Differentiation of Hepatic Progenitor Cells via Wnt Signaling Pathway

Figure 2

Induction of autophagy suppressed hepatic differentiation of HPCs. (a) Western blot showing the level of LC3-II/LC3-I and P62 in WB-F344 cells treated with differentiation medium (HGF) for 2 days and coculture DMSO or rapamycin (200 nM; Rapa) for 24 h. (b) RT-PCR analyzing the expression of hepatic lineage markers (Alb and Aldob) in WB-F344 cells treated with differentiation medium (HGF) for 2 days and coculture DMSO or rapamycin (200 nM; Rapa) for 24 h. (c) Western blot showing the expression of hepatic lineage markers (Alb and Aldob) in WB-F344 cells treated with differentiation medium (HGF) for 2 days and coculture DMSO or rapamycin (200 nM; Rapa) for 24 h. (d) Western blot showing the level of LC3-II/LC3-I and P62 in WB-F344 cells treated with differentiation medium (HGF) for 2 days and coculture DMSO or starvation (serum-free medium; Starve) for 24 h. (e) RT-PCR analyzing the expression of hepatic lineage markers (Alb and Aldob) in WB-F344 treated with differentiation medium (HGF) for 2 days and coculture DMSO or starvation (serum-free medium; Starve) for 24 h. (f) Western blot showing the expression of hepatic lineage markers (Alb and Aldob) in WB-F344 treated with differentiation medium (HGF) for 2 days and coculture DMSO or starvation (serum-free medium; Starve) for 24 h. Data represent ; . .

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