Autophagy Is Required for Hepatic Differentiation of Hepatic Progenitor Cells via Wnt Signaling Pathway

Figure 3

Wnt signaling pathway was involved during hepatic differentiation of HPCs. (a) RT-PCR analyzing the mRNA expression of Wnt1, Wnt3a, Wnt4, Wnt5a, and Wnt9a in WB-F344 cells treated with hepatic differentiation medium (HGF) for 4 days. (b) RT-PCR analyzing the mRNA expression of β-catenin, Sox9, and Axin2 in WB-F344 cells treated with hepatic differentiation medium (HGF) for 4 days. (c) Western blot showing the level of Wnt5a, P-CaMKIIα, and CaMKIIα in WB-F344 cells treated with hepatic differentiation medium (HGF) for 4 days. (d) Western blot showing the level of Wnt3a, β-catenin, and Sox9 in WB-F344 cells treated with hepatic differentiation medium (HGF) for 4 days. Data represent ; ; . .

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